Vectors and Constructs

Targeting/Transgenic Vector Construction

Using BAC recombineering, we can generate constructs in as little as 6 weeks from initial consultation to final sequencing verification. By performing restriction analyses verification at key steps of the process, we have increased the quality and reliability, as well as the speed, of vector construction. We can create BAC transgenic constructs for uncharacterized promoters. This technique allows insertion of large (>20 kb) genomic sequences from BACs into smaller vectors suitable for gene targeting. This process bypasses the restraints of conventional ligations to produce longer genomic “arms” to increase the efficiency of homologous recombination in ES cells. Moreover, PCR error in these arms is circumvented since genomic DNA in the BAC clones is manipulated to generate the DNA constructs.  Site-directed mutagenesis, point mutations, or any kind of Knock-In constructs pose no problems for this technology.  We have the capacity and flexibility to tailor-make elements for targeting and transgenic constructs (including BAC transgenic).  Additionally, we are currently working at implementing a yeast system to perform many steps even more rapidly and reliably.

Rates and Timelines

We charge $4500 for vector construction to University of California Investigators ($6500 for all other academic investigators).  This price is negotiable for constructs that require fewer steps.  Our timeframe for completion is 3-6 months.  Most KO, conditional KO and Knock-In constructs are completed in 3 months.  We do not charge until completion of the construct.

Make a custom construct

Library of available cassettes

Future goals